Najam A. Sharif

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Research Protocols for Ophthalmic Disease Mechanisms and Therapeutics: Glaucoma – Ocular Hypertension offers a detailed, step-by-step protocols for isolating, characterizing, and analyzing cells and tissues of the eye’s anterior segment—where OHT originates—using techniques from molecular biology, pharmacology, and histology. Across eight thematic sections, the book covers ocular anatomy, drug screening, receptor localization, cell-based assays, in vitro and in vivo models, drug formulation, and cutting-edge technologies such as gene editing, single-cell RNA sequencing, and stem cell therapies. Each protocol is designed to support reproducibility and translational relevance. Key Features: -Details validated protocols for drug discovery and disease modeling -Demonstrates methods for studying receptor function and pharmacokinetics -Provides animal models and ex vivo systems to evaluate therapeutic strategies -Incorporates emerging tools such as CRISPR, metabolomics, and exosomes -Bridges experimental design with clinical relevance for glaucoma research